Catalog Number - 68504-SP250

Cogent RP C4™ is a reversed-phase HPLC stationary phase designed for the purification and isolation of hydrophobic and neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this stationary phase provides reproducible chromatographic performance and lower hydrophobic retention than C8 phases. The chemistry is particularly useful for semi-preparative applications where compound recovery, increased loading capacity, and maximum separation efficiency are required.

68504-SP250 is a semi-preparative HPLC column packed with 5 µm RP C4™ particles featuring a 100 Å pore size in a 10 mm ID x 250 mm format. The 10 mm internal diameter provides substantially greater sample loading capacity than analytical columns, while the 250 mm length delivers maximum chromatographic resolution and peak capacity within the semi-preparative product family. Typical operating flow rates are approximately 4–8 mL/min depending on mobile phase composition, analyte properties, and system capabilities.

This column is particularly useful for purification of hydrophobic and neutral compounds in pharmaceutical, chemical, and research laboratories. Compared to C8 and C18 phases, the C4 bonded phase provides lower retention, which can improve recovery and reduce purification cycle times. The 250 mm length is ideal for difficult separations requiring maximum resolving power and purification efficiency. A semi-preparative HPLC system capable of the required flow rates is recommended for optimal performance.

Key Benefits

• RP C4™ stationary phase

• USP L26 equivalent chemistry

• Butyl (C4) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• Lower retention than C8 phases

• Increased sample loading capacity

• Excellent for compound purification

• 5 µm particle size

• Maximum semi-preparative resolution

Typical Applications

• Semi-preparative HPLC

• Compound purification

• Fraction collection

• Pharmaceutical purification

• Hydrophobic compound purification

• Neutral compound purification

• Method development

• Process development

• Research and development

• Sample isolation

Specifications

• Product Type: Semi-Preparative HPLC Column

• Stationary Phase: RP C4™

• USP Classification: L26

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 10 mm ID x 250 mm

• Length: 250 mm

• Typical Flow Rate: 4–8 mL/min

• Brand: Cogent

Special Order Item Information

• Please review the Special Order Item Information for complete details.

• For pricing confirmation, availability, lead times, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for purification of hydrophobic and neutral compounds

• Provides less retention than C8 stationary phases

• Ideal for semi-preparative purification and fraction collection

• The 10 mm ID format offers increased loading capacity compared to analytical columns

• Typical operating flow rates are approximately 4–8 mL/min

• 5 µm particles provide lower operating backpressure and robust performance

• Fully double end capped to minimize secondary interactions

• The 250 mm length provides maximum resolution and peak capacity for challenging purifications

• Requires an HPLC system capable of semi-preparative flow rates

• This is a Special Order Item